人類體外檢測麻疹病毒診斷試劑盒
| 型 號： | 美國FOCUS |
| 報 價： |  |
人類體外檢測麻疹病毒診斷試劑盒：麻疹是兒童Z常見的急性呼吸道傳染病之一，其傳染性很強，如需了解詳情請廣州健侖生物科技有限公司
- 產(chǎn)品描述
人類體外檢測麻疹病毒診斷試劑盒
英文名稱：American FUCUS measles virus diagnostic kit
廣州健侖生物科技有限公司
（廣州健侖生物科技有限公司是集研制開發(fā)、銷售、服務(wù)于一體的優(yōu)良企業(yè),公司產(chǎn)品涉及臨床快速診斷試劑、食品安全檢測試劑，違禁品快速檢測，動物疾病防疫檢測試劑，免疫診斷試劑、臨床血液學(xué)和體液學(xué)檢驗試劑、微生物檢驗試劑、分子生物學(xué)檢驗試劑、臨床生化試劑、有機試劑等眾多領(lǐng)域，同時核心代理Panbio、FOCUS、Qiagen、IBL、CORTEZ、Fuller、Inbios、BinaxNOW、LumuQuick、日本富士、日本生研等多家有名診斷產(chǎn)品集團公司產(chǎn)品，致力于為商檢單位、疾病預(yù)防控制中心、海關(guān)出入境檢疫局、衛(wèi)生防疫單位,緝毒系統(tǒng)，戒毒中心，檢驗檢疫單位、生化企業(yè)、科研院所、醫(yī)療機構(gòu)等機構(gòu)與行業(yè)提供*、高品質(zhì)的產(chǎn)品服務(wù)。此外，本公司還開展食品、衛(wèi)生、環(huán)境、藥品等多方面的第三方檢測服務(wù)。）
主要用途：用于定量測定人血清、腦脊液或血漿中的麻疹抗體。
產(chǎn)品規(guī)格：96T/盒
存儲條件：4-8℃
保質(zhì)期：18個月
【酶聯(lián)免疫法的原理】
①使抗原或抗體結(jié)合到某種固相載體表面，并保持其免疫活性。
②使抗原或抗體與某種酶連接成酶標(biāo)抗原或抗體，這種酶標(biāo)抗原或抗體既保留其免疫活性，又保留酶的活性。在測定時，把受檢標(biāo)本（測定其中的抗體或抗原）和酶標(biāo)抗原或抗體按不同的步驟與固相載體表面的抗原或抗體起反應(yīng)。用洗滌的方法使固相載體上形成的抗原抗體復(fù)合物與其他物質(zhì)分開，*后結(jié)合在固相載體上的酶量與標(biāo)本中受檢物質(zhì)的量成一定的比例。加入酶反應(yīng)的底物后，底物被酶催化變?yōu)橛猩a(chǎn)物，產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān)，故可根據(jù)顏色反應(yīng)的深淺來進行定性或定量分析。由于酶的催化頻率很高，故可極大地放大反應(yīng)效果，從而使測定方法達到很高的敏感度。ELISA可用于測定抗原，也可用于測定抗體。
① antigen or antibody to a solid surface of the carrier, and maintain its immunocompetence.
② the antigen or antibody connected with an enzyme labeled antigen or antibody, this enzyme-labeled antigen or antibody retains both its immunological activity, but also retains the enzyme activity. In the measurement, the test sample (measured in the antibody or antigen) and the enzyme-labeled antigen or antibody in different steps with the solid phase carrier antigen or antibody reaction. The antigen-antibody complex formed on the solid phase carrier is separated from other substances by the washing method. * The amount of enzyme bound to the solid phase carrier is proportional to the amount of the test substance in the specimen. After adding enzyme reaction substrate, the substrate is enzymatically converted into a colored product. The amount of the product is directly related to the amount of the tested substance in the sample. Therefore, qualitative or quantitative analysis can be conducted according to the color reaction depth. Due to the high catalytic frequency of the enzyme, it is possible to greatly amplify the reaction effect so that the determination method achieves a high sensitivity. ELISA can be used to determine the antigen can also be used to determine the antibody.
【檢測原理】
The immunological processes involved in ELISA (enzyme-linked immunosorbent assay) are especially confirmed in the area of infection testing for antibodies. This detection reaction is based on the specific interaction of antibodies and antigens. To this end, binding antibodies coated in the patient's sample are present in test strips of microtitre plates of Cyurotype ELISA classic. Other alkaline phosphatase secondary antibody was used to detect the resulting immune complexes. The enzyme catalyzes a reaction, the conversion of p-nitrophenol in the colored product of the colorless substrate p-nitrophenylphosphate. The signal intensity of the reaction product is detected photometrically proportional to the concentration of antibody in the sample.
人類體外檢測麻疹病毒診斷試劑盒
【試劑盒的組成】
試劑盒組成 | IgG試劑盒 IgM試劑盒 IgA試劑盒 數(shù)量 / 容積 |
微孔條（此微孔條可拆下單獨使用，每條有8孔，共96孔，已經(jīng)包被了抗原） 1個微孔條框架 包被材料未被激活 | 12 12 12 |
標(biāo)準(zhǔn)血清（立即可用） 人血清溶于含蛋白的磷酸鹽緩沖液；抗HIV抗體、抗乙肝病毒（HBV）表面抗原和抗丙肝病毒（HCV）抗體均為陰性； 防腐劑：< 0.1% * 染色劑：紫紅色O | 2×2毫升 2×2毫升 2×2毫升 |
陰性對照血清（立即可用） 人血清溶于含蛋白的磷酸鹽緩沖液；抗HIV抗體、抗乙肝病毒（HBV）表面抗原和抗丙肝病毒（HCV）抗體均為陰性； 防腐劑：< 0.1% * 染色劑：里沙明綠 V | 1×2毫升 1×2毫升 1×2毫升 |
酶標(biāo)記的抗人IgG, IgA, IgM （立即可用） 羊抗人IgG, IgA, IgM（多克隆），標(biāo)記堿性磷酸酶后在蛋白穩(wěn)定劑中儲存 防腐劑: 0.01% 甲基異噻唑啉酮 0.01% 溴化硝基二堊烷 | 13毫升 13毫升 13毫升 |
濃縮洗液（可稀釋至1000毫升） 氯化鈉溶液，含吐溫20和30mM Tris 防腐劑： < 0.1%* | 1×33.3毫升 1×33.3毫升 1×33.3毫升 |
稀釋緩沖液 磷酸鹽緩沖液，內(nèi)含蛋白和吐溫20 防腐劑： < 0.1%* 0.01克 /升的溴酚藍鈉鹽 | 2×50毫升 2×50毫升 2×50毫升 |
終止液 1.2N 氫氧化鈉 | 15毫升 15毫升 15毫升 |
底物（立即可用） 對硝基苯磷酸鹽，不含其它溶劑的緩沖液 防腐劑：< 0.1% * （未開封瓶子中的底物可能會輕微變黃，但不會影響其質(zhì)量） | 13毫升 13毫升 13毫升 |
帶有標(biāo)準(zhǔn)曲線和評估表的質(zhì)量控制文件 （抗體以IU/毫升或U/毫升計量） | 1 1 1 |
我司同時還提供、美國FOCUS、西班牙DIA、美國trinity等試劑盒：
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Capsules Many bacterial cell walls surround a thick layer of thick, jelly-like material. The capsule of most bacteria (such as pneumococcal, meningococcal, etc.) consists of polysaccharides. In addition to the capsule to help identify bacteria, but also protect the bacteria from phagocytic phagocytosis and digestion, and thus the toxicity of bacteria. The mechanism of anti-phagocytic capsule is not very clear, probably due to the capsule mucus layer is relatively smooth, easy to capture by phagocytes. The capsule can store moisture to make the bacteria resistant to drying, and have some resistance to the infringement of other factors (such as lysozyme, complement, antibodies, antibacterials, etc.).
2. Flagella (Flagllum) In some bacterial cells with elongated and curved filaments, called flagella. Flagella are bacterial organs of movement, tend to have chemical tendencies, often toward a high concentration of nutrients in the direction of moving away from its harmful environment.
3. Pilus Pilus is a filamentous appendage that is thinner, shorter, straighter, harder, and more numerous than flagella over the surface of many Gram-negative bacteria, also known as Fimbriae. Pili has nothing to do with exercise. Pili can be divided into common pili (Commonpilus) and sexual pili (Sexpilus) two.
4. Spore (Bacillus) under certain conditions, Bacillus (such as Bacillus anthracis) and Clostridium (such as tetanus, gas gangrene pathogen) in the body to form a highly refractories difficult to color small Body, called the endospore (Endospore), referred to as spores.
5. Bacteria can be reproductive asexual or genetic recombination in two ways, the bacteria are generally a simple two-division method of asexual reproduction, individual bacteria, such as Mycobacterium tuberculosis occasionally branch propagation. Aseptic propagation of the two fissions, the salient features of its breeding fast. The generation of E. coli (the necessary time for bacterial doubling doubling) is 20 minutes, calculated in accordance with this, under the best conditions after 8 hours, a cell can proliferate to 2 million, after 10 hours can exceed 1 billion, 24 hours After that, the number of bacterial multiplication can be huge enough to count the data and extent.
6. Individual cells also undergo genetic variation in several ways: mutations (where the genetic code of the cell itself changes randomly), transformation (transfer of bacterial DNA from one bacterium to another bacterium in solution), transfection (virus Or bacterial DNA, or DNA from both, by phage transfer into another bacterium), bacterial conjugation (the DNA of one bacterium binds to another bacterium by the special protein structure formed between the two bacteria, which binds the pili) . Bacteria can get DNA in these ways, then divide and pass on the recombinant genome to offspring. Many bacteria contain plasmids that contain extrachromosomal DNA.
